ABSTRACT
BACKGROUND:In Burkina Faso; there is no recent data about the level of drug resistance in Mycobacterium tuberculosis strains among newly diagnosed tuberculosis cases. OBJECTIVE: To provide an update of the primary drug resistance of mycobacterium tuberculosis among patients in Burkina faso. METHODS: Mycobacterium strains were identified in 323 newly diagnosed tuberculosis patients between April 2005 and September 2006; and their susceptibility to isoniazid; rifampicin; streptomycin; and ethambutol was determined according to the proportions method. Among these patients; 243 accepted voluntarily to be tested for antibodies to HIV. RESULTS: The age range of the patients was 11 and 75 years and included 221 (68.4) males and 102 (21.6) females. The isolates included 314 (97.2) M. tuberculosis; eight (0.3) M. africanum and one M. bovis. Thirty-nine (12.4) of the M. tuberculosis strains were resistant; with 7.3resistant to one drug; 2.9to two drugs; 0.3to three drugs and 1.9to four drugs. In total 3.2of the isolates were multidrug-resistant (MDR). One isolate of M. africanum was resistant to all drugs while the single strain of M. bovis was sensitive to all the drugs. Among the 243 patients tested for HIV 77 were positive. However; there was no relationship between drug resistance and gender; age group or HIV serostatus of the patients. CONCLUSION: The resistance rate of M. tuberculosis strains to all the four drugs tested (12.4) and the rate of MDR (3.2) are high. These results demand an increased effort by the National Tuberculosis Program to limit the spread of MDR strains of tuberculosis
Subject(s)
Drug Resistance , Mycobacterium tuberculosis , TuberculosisABSTRACT
Background: Tuberculosis drug-resistance becomes common in sub-Saharan Africa; however; very few data are available in Burkina Faso. The aim of this study is to assess the acquired resistance of Mycobacterium tuberculosis complex strains identified in TB patients to four first-line drugs in Ouagadougou. Methods: One hundred and ten (110) pulmonary tuberculosis patients with acid-fast bacilli-positive sputum and in situation of failure; relapse; or treatment abandonment were included in the study. Ninety six strains; including 92 (95.8) M. tuberculosis and 4 (4.2) M. africanum; were isolated from the sputum samples of these patients. Their drug susceptibility testing was performed using the proportion method. The first-line drugs tested were isoniazid (INH); streptomycin (STR); ethambutol (EMB); and rifampicin (RIF). Results: The overall drug-resistance rate of M. tuberculosis was 67.4(n=60); including 3.4to one drug; 18to two; 10.1to three; and 35.9to four drugs. The resistance to INH; RIF; EMB; and STR were 67.4; 51.7; 50.6; and 44.9; respectively. Two strains of M. africanum were resistant to all drugs. Forty-six (51.7) strains were multidrug-resistant (resistant to at least INH and RIF). Conclusions: In previously treated patients; the level of resistance of M. tuberculosis complex to commonly used anti-tuberculosis drugs is very high in Ouagadougou. Our results showed that multidrug-resistant tuberculosis could be a public health problem in Burkina Faso